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Direct inoculation or membrane filtration, 14d at 20-25°C & 30-35°C
Acid/heat treatment, BCYE agar culture, 10d at 35°C
Growth promotion with 5 strains on TSA plates
Bacteriostasis/fungistasis test with 6 challenge organisms
Method suitability study for product-specific sterility testing
Decontamination, concentration, culture on Löwenstein-Jensen
Growth promotion test with C.albicans, A.brasiliensis on SDA
Selective enrichment at 42°C, microaerophilic conditions