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Direct inoculation or membrane filtration, 14d at 20-25°C & 30-35°C
Extraction, filtration, TSA incubation 3-5d at 30-35°C, aerobic conditions
ICP-MS analysis for heavy metals suite
Simulated use extraction, GC-FID headspace analysis
Particle extraction, SEM imaging + EDX elemental mapping
Direct LAL test on water sample
GC-MS extraction method
Extraction in particle-free water, light obscuration counting
Recovery efficiency + method suitability + 2 ID of dominants
Additional extraction cycle for EO/ECH
Additional SEM-EDX analysis on subsequent samples
Membrane filtration, TSA plate count, 3d at 30-35°C
Growth promotion with 5 strains on TSA plates
Method validation with count standards at 10µm and 25µm
Extraction/rinse, filtration, TSA 3-5d & SDA 5-7d incubation
Extraction in DMF, GC-FID quantification
Membrane filtration, R2A agar incubation 7d at 20-25°C
Rabbit intradermal injection of polar/non-polar extracts
Bacteriostasis/fungistasis test with 6 challenge organisms
Interference test at 3 dilutions with spike recovery
Anaerobic incubation on TSA or FTM media, 5-7 days
Direct measurement at 25°C with conductivity cell
Rabbit pyrogen test, saline extract, temperature monitoring
Method suitability study for product-specific sterility testing
Screening at multiple dilutions to find valid test range
Growth promotion test with C.albicans, A.brasiliensis on SDA
Recovery test with B.subtilis spike on products
Direct pH measurement at 25°C with calibrated electrode
GLP-compliant rabbit pyrogen test with saline extract
Direct water analysis by combustion/oxidation method
Kinetic chromogenic LAL assay, 0.005-5.0 EU/ml range